Expression of GFP and RFP in HEK293 and ExpiCHO cells was measured 72 hours No cells survived medium or highly stringent selection conditions. Protein Expression Optimization.
Fig. 2. FectoPRO® enables faster protein production in ExpiCHO™ system. ExpiCHO-S™ cells were seeded following the recommended protocol in ExpiCHO™ Expression Medium, and transfected with FectoPRO® (0.8 μg DNA/mL) or ExpiFectamine™ CHO + Feed + Enhancer (0.8 μg DNA/mL) following the standard protocols.
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Lowmaster’s Presentation Researchers are demanding higher titers in transient systems, and many people use CHO systems as an alternative to HEK293 cells. The Thermo Fisher Gibco ExpiCHO Expression System kit was developed for transient expressing…
Includes: 2 vials frozen ExpiCHO-S™ cells, 1 bottle 1000mL ExpiCHO™ Expression Medium, 1 ExpiFectamine™ CHO transfection kit for 1 L of culture, 1 vial Antibody Expressing Positive Control Vector, 1 bottle 100mL OptiPRO™ SFM; store all components at +2° - +8° C, except frozen cells stored in liquid nitrogen
Stable expression clones using codon optimized open reading frame were developed in human 293-H cells with yields reaching ~ 25 mg of GP1 protein per liter of spent medium. 4:30 Using GlycoExpress to Overcome Production Limitations for Difficult-to-Express Proteins
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electroporated using the MaxCyte STX with GFP plasmid. All cells, including ExpiCHO cells were cultured in commercially available medium. ExpiCHO transfected cells were cultured in manufacturer recommended medium. 24 hours post electroporation, GFP expression was assessed via FACS analysis. CHOZN® DG44 Derivative CHO3e7 CHOK1-SV CHO-S ExpiCHO